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envelope plasmid vsv g  (Addgene inc)


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    Addgene inc envelope plasmid vsv g
    Envelope Plasmid Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/envelope+plasmid+pcag+vsvg/pCAG-VSVG+(Plasmid+%2335616)/pmc12340043-84-18-23
    Average 93 stars, based on 42 article reviews
    envelope plasmid vsv g - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: A universal reporter cell line for bioactivity evaluation of engineered cytokine products
    Article Snippet: .. They were then transiently co-transfected with the reporter plasmid pJM046 as well as the packaging plasmid psPAX2 (Addgene, #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) and the envelope plasmid pCAG-VSVG (Addgene, #35616; http://n2t.net/addgene:35616 ; RRID:Addgene_35616) using the jetPRIME reagent (Polyplus transfection). ..

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models.
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc (28). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc ( ). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 μg packaging plasmid psPAX2 (Addgene 12260), 0.9 μg envelope plasmid pCAG-VSVG (Addgene 35616), 9 μg scramble control or shLDHB vectors (Origene TL311768) in 225 μL Opti-MEM (Thermo Fischer 31985062) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8 μg/mL polybrene for 72 h. Cells were then selected with 1–2 μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: Assessing the metastatic potential of circulating tumor cells using an organ-on-chip model
    Article Snippet: .. In detail, VSV-G pseudotyped lentivirus was produced by transfection of 293T cells (Invitrogen, Waltham, MA, U.S.) using Lipofectamine 2000, 9 μg packaging plasmid psPAX2 (Cat. #12260, Addgene, Watertown, MA, U.S.), 0.9 μg envelope plasmid pCAG-VSVG (Cat. #35616, Addgene, Watertown, MA, U.S.), 9 μg LV-GFP (Cat. #25999, Addgene, Watertown, MA, U.S.) or LV-RFP (Cat. #26001, Addgene, Watertown, MA, U.S.) in 225 μL Opti-MEM I (Cat. #31985062, Thermo Fisher Scientific, Waltham, MA, U.S.) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000 (Cat. 11668019, Thermo Fisher Scientific, Waltham, MA, U.S.). ..

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9μg packaging plasmid psPAX2 (Addgene 12260), 0.9μg envelope plasmid pCAG-VSVG (Addgene 35616), 9μg scramble control or shLDHB vectors (Origene TL311768) in 225μL Opti-MEM (Thermo Fischer 31985062) mixed with 90μL Opti-MEM containing 54μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8μg/mL polybrene for 72 h. Cells were then selected with 1–2μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wildtype p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK-293T (RRID:CVCL_0063) were transfected with the lentiviral plasmid 6 encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 7 (RRID:Addgene_12260) and envelope plasmid pCAG-VSVG (RRID:Addgene_64084) (both 8 kindly provided by Dr. Patrick Salmon, University of Geneva, Switzerland). .. Transfection was 9 performed using JetPRIME ® transfection reagent (Brunschwig Lab supplies, 114-15) according 10 to manufacturer’s recommendations.

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wild-Type p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK293T cells (RRID: CVCL_0063) were transfected with the LV plasmid encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 (RRID: Addgene_12260) and envelope plasmid pCAG-VSVG (RRID: Addgene_64084; both kindly provided by Dr. Patrick Salmon, University of Geneva, Geneva, Switzerland). .. Transfection was performed using JetPRIME transfection reagent (Brunschwig Lab supplies, 114-15) according to the manufacturer’s recommendations.

    Generated:

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models.
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc (28). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc ( ). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Transfection:

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models.
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc (28). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Article Title: Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models
    Article Snippet: Raji cells (ATCC) were engineered to express luciferase (Luc) and green fluorescent protein (GFP) with the use of the bidirectional lentiviral vector pSMAL-Luc ( ). .. In brief, lentiviruses were generated by transfection of 293T cells with pSMAL-Luc together with the envelope plasmid pCAG-VSVG (Addgene) and the packaging plasmid psPAX2 (Addgene) and with the use of the Lipofectamine 2000 reagent (Life Technologies). .. The viruses were concentrated with the use of a Lenti-X concentrator (Clontech) and stored at –80°C until use.

    Article Title: Assessing the metastatic potential of circulating tumor cells using an organ-on-chip model
    Article Snippet: .. In detail, VSV-G pseudotyped lentivirus was produced by transfection of 293T cells (Invitrogen, Waltham, MA, U.S.) using Lipofectamine 2000, 9 μg packaging plasmid psPAX2 (Cat. #12260, Addgene, Watertown, MA, U.S.), 0.9 μg envelope plasmid pCAG-VSVG (Cat. #35616, Addgene, Watertown, MA, U.S.), 9 μg LV-GFP (Cat. #25999, Addgene, Watertown, MA, U.S.) or LV-RFP (Cat. #26001, Addgene, Watertown, MA, U.S.) in 225 μL Opti-MEM I (Cat. #31985062, Thermo Fisher Scientific, Waltham, MA, U.S.) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000 (Cat. 11668019, Thermo Fisher Scientific, Waltham, MA, U.S.). ..

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wildtype p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK-293T (RRID:CVCL_0063) were transfected with the lentiviral plasmid 6 encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 7 (RRID:Addgene_12260) and envelope plasmid pCAG-VSVG (RRID:Addgene_64084) (both 8 kindly provided by Dr. Patrick Salmon, University of Geneva, Switzerland). .. Transfection was 9 performed using JetPRIME ® transfection reagent (Brunschwig Lab supplies, 114-15) according 10 to manufacturer’s recommendations.

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wild-Type p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK293T cells (RRID: CVCL_0063) were transfected with the LV plasmid encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 (RRID: Addgene_12260) and envelope plasmid pCAG-VSVG (RRID: Addgene_64084; both kindly provided by Dr. Patrick Salmon, University of Geneva, Geneva, Switzerland). .. Transfection was performed using JetPRIME transfection reagent (Brunschwig Lab supplies, 114-15) according to the manufacturer’s recommendations.

    Produced:

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 μg packaging plasmid psPAX2 (Addgene 12260), 0.9 μg envelope plasmid pCAG-VSVG (Addgene 35616), 9 μg scramble control or shLDHB vectors (Origene TL311768) in 225 μL Opti-MEM (Thermo Fischer 31985062) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8 μg/mL polybrene for 72 h. Cells were then selected with 1–2 μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: Assessing the metastatic potential of circulating tumor cells using an organ-on-chip model
    Article Snippet: .. In detail, VSV-G pseudotyped lentivirus was produced by transfection of 293T cells (Invitrogen, Waltham, MA, U.S.) using Lipofectamine 2000, 9 μg packaging plasmid psPAX2 (Cat. #12260, Addgene, Watertown, MA, U.S.), 0.9 μg envelope plasmid pCAG-VSVG (Cat. #35616, Addgene, Watertown, MA, U.S.), 9 μg LV-GFP (Cat. #25999, Addgene, Watertown, MA, U.S.) or LV-RFP (Cat. #26001, Addgene, Watertown, MA, U.S.) in 225 μL Opti-MEM I (Cat. #31985062, Thermo Fisher Scientific, Waltham, MA, U.S.) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000 (Cat. 11668019, Thermo Fisher Scientific, Waltham, MA, U.S.). ..

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9μg packaging plasmid psPAX2 (Addgene 12260), 0.9μg envelope plasmid pCAG-VSVG (Addgene 35616), 9μg scramble control or shLDHB vectors (Origene TL311768) in 225μL Opti-MEM (Thermo Fischer 31985062) mixed with 90μL Opti-MEM containing 54μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8μg/mL polybrene for 72 h. Cells were then selected with 1–2μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Control:

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 μg packaging plasmid psPAX2 (Addgene 12260), 0.9 μg envelope plasmid pCAG-VSVG (Addgene 35616), 9 μg scramble control or shLDHB vectors (Origene TL311768) in 225 μL Opti-MEM (Thermo Fischer 31985062) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8 μg/mL polybrene for 72 h. Cells were then selected with 1–2 μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9μg packaging plasmid psPAX2 (Addgene 12260), 0.9μg envelope plasmid pCAG-VSVG (Addgene 35616), 9μg scramble control or shLDHB vectors (Origene TL311768) in 225μL Opti-MEM (Thermo Fischer 31985062) mixed with 90μL Opti-MEM containing 54μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8μg/mL polybrene for 72 h. Cells were then selected with 1–2μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Incubation:

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 μg packaging plasmid psPAX2 (Addgene 12260), 0.9 μg envelope plasmid pCAG-VSVG (Addgene 35616), 9 μg scramble control or shLDHB vectors (Origene TL311768) in 225 μL Opti-MEM (Thermo Fischer 31985062) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8 μg/mL polybrene for 72 h. Cells were then selected with 1–2 μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9μg packaging plasmid psPAX2 (Addgene 12260), 0.9μg envelope plasmid pCAG-VSVG (Addgene 35616), 9μg scramble control or shLDHB vectors (Origene TL311768) in 225μL Opti-MEM (Thermo Fischer 31985062) mixed with 90μL Opti-MEM containing 54μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8μg/mL polybrene for 72 h. Cells were then selected with 1–2μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Virus:

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 μg packaging plasmid psPAX2 (Addgene 12260), 0.9 μg envelope plasmid pCAG-VSVG (Addgene 35616), 9 μg scramble control or shLDHB vectors (Origene TL311768) in 225 μL Opti-MEM (Thermo Fischer 31985062) mixed with 90 μL Opti-MEM containing 54 μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8 μg/mL polybrene for 72 h. Cells were then selected with 1–2 μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Article Title: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer.
    Article Snippet: .. Lentivirus was produced in 293T cells using Lipofectamine 2000 (9μg packaging plasmid psPAX2 (Addgene 12260), 0.9μg envelope plasmid pCAG-VSVG (Addgene 35616), 9μg scramble control or shLDHB vectors (Origene TL311768) in 225μL Opti-MEM (Thermo Fischer 31985062) mixed with 90μL Opti-MEM containing 54μL Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later. .. The lentiviruses were used to infect cells with 8μg/mL polybrene for 72 h. Cells were then selected with 1–2μg/mL puromycin (Sigma-Aldrich P8833-25MG) for 72 h. The inhibition of LDHB expression was verified by Western blot.

    Sequencing:

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wildtype p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK-293T (RRID:CVCL_0063) were transfected with the lentiviral plasmid 6 encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 7 (RRID:Addgene_12260) and envelope plasmid pCAG-VSVG (RRID:Addgene_64084) (both 8 kindly provided by Dr. Patrick Salmon, University of Geneva, Switzerland). .. Transfection was 9 performed using JetPRIME ® transfection reagent (Brunschwig Lab supplies, 114-15) according 10 to manufacturer’s recommendations.

    Article Title: The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wild-Type p53 to Exert the Dominant-Negative Effect
    Article Snippet: .. In brief, HEK293T cells (RRID: CVCL_0063) were transfected with the LV plasmid encoding the DNA sequence of interest in combination with the packaging plasmid psPAX2 (RRID: Addgene_12260) and envelope plasmid pCAG-VSVG (RRID: Addgene_64084; both kindly provided by Dr. Patrick Salmon, University of Geneva, Geneva, Switzerland). .. Transfection was performed using JetPRIME transfection reagent (Brunschwig Lab supplies, 114-15) according to the manufacturer’s recommendations.



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